Dnmt1 CLIA Kit for Mouse(Mus musculus) | EIAab.comDnmt1 CLIA Kit for Mouse(Mus musculus)

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Dnmt1 (Gene name),

DNA (cytosine-5)-methyltransferase 1 (Protein name ), DNMT1_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Dnmt1(Dnmt;Met1;Uim);

Protein name:

DNA (cytosine-5)-methyltransferase 1(Dnmt1;Met-1);

Alternative:

MCMT;DNA methyltransferase MmuI(DNA MTase MmuI;M.MmuI);

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Homodimer. Interacts with HDAC1 and with PCNA. Binds MBD2 and MBD3. Component of complexes containing SUV39H1. Interacts with DNMT3A and DNMT3B (By similarity). Forms a complex with DMAP1 and HDAC2, with direct interaction. Interacts with the PRC2/EED-EZH2 complex. Interacts with UBC9 (By similarity). Interacts with BAZ2A/TIP5.

Function:

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.

Subcellular Location:

Nucleus Cytoplasm It is nucleoplasmic through most of the cell cycle and associates with replication foci during S-phase. In germ cells, spermatogonia, preleptotene and leptotene spermatocytes all express high levels of nuclear protein, while the protein is not detected in pachytene spermatocytes, despite the fact they expressed high levels of mRNA. In females, the protein is not detected in non-growing oocytes, in contrast to the growing oocytes. During the growing, the protein is no longer detectable in nuclei but accumulates to very high levels first throughout the cytoplasm. At the time of ovulation, all the protein is cytoplasmic and is actively associated with the oocyte cortex. After fecondation, in the preimplantation embryo, the protein remains cytoplasmic and after implantation, it is exclusively nuclear in all tissue types. Isoform 2 is sequestered in the cytoplasm of maturing oocytes and of preimplantation embryos, except for the 8-cell stage, while isoform 1 is exclusively nuclear.

Protein Attributes:

Sequence length:

1620

Sequence:

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1600:

1620:

SARESASAAV | KAKEEAATKD |

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF01426

UniGene:

Mm.128580

String:

P13864

SMR:

P13864

KEGG:

mmu:13433

Uniprot:

P13864

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ELISA Kit for Mouse Dnmt1

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E2102h

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ELISA Kit for Mouse Dnmt1

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E2102c

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ELISA Kit for Mouse Dnmt1

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E2102m

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ELISA Kit for Mouse Dnmt1

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E2102b

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ELISA Kit for Mouse Dnmt1

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E2102r

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96T

CLIA Kit for Mouse Dnmt1

Cat.:

U2102m

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96T

CLIA Kit for Mouse Dnmt1

Cat.:

U2102h

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96T

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CLIA Kit for Mouse Dnmt1

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U2102c

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96T

CLIA Kit for Mouse Dnmt1

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U2102r

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CLIA Kit for Mouse Dnmt1

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U2102b

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Polyclonal Antibody for Mouse Dnmt1

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P2102Rb-h

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40ug/0.2ml

Polyclonal Antibody for Mouse Dnmt1

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P2102Rb-r

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Polyclonal Antibody for Mouse Dnmt1

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P2102Rb-m

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Polyclonal Antibody for Mouse Dnmt1

Monoclonal Antibody for Mouse Dnmt1

Protein for Mouse Dnmt1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

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Recovery

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Linearity

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References

1.

"Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells. The carboxyl-terminal domain of the mammalian enzymes is related to bacterial restriction methyltransferases."
Bestor T.H. , Laudano A. , Mattaliano R. , Ingram V.
J. Mol. Biol.203:971-983(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
2.

"New 5' regions of the murine and human genes for DNA (cytosine-5)-methyltransferase."
Yoder J.A. , Yen R.-W.C. , Vertino P.M. , Bestor T.H. , Baylin S.B.
J. Biol. Chem.271:31092-31097(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION TO N-TERMINUS
tissue: Embryo.
3.

"Expression of an alternative Dnmt1 isoform during muscle differentiation."
Aguirre-Arteta A.M. , Grunewald I. , Cardoso M.C. , Leonhardt H.
Cell Growth Differ.11:551-559(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
strain: C57BL/6.
tissue: Skeletal muscle.
4.

"Structure and function of the mouse DNA methyltransferase gene: Dnmt1 shows a tripartite structure."
Margot J.B. , Aguirre-Arteta A.M. , Di Giacco B.V. , Pradhan S. , Roberts R.J. , Cardoso M.C. , Leonhardt H.
J. Mol. Biol.297:293-300(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2)
strain: C57BL/6.
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
strain: C57BL/6.
tissue: Brain.
6.

"Sex-specific exons control DNA methyltransferase in mammalian germ cells."
Mertineit C. , Yoder J.A. , Taketo T. , Laird D.W. , Trasler J.M. , Bestor T.H.
Development125:889-897(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 1-27 AND 119-1619 (ISOFORMS 1 AND 2)
7.

"A short DNA methyltransferase isoform restores methylation in vivo."
Gaudet F. , Talbot D. , Leonhardt H. , Jaenisch R.
J. Biol. Chem.273:32725-32729(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE OF 1-144 (ISOFORMS 1 AND 2);PROTEIN SEQUENCE OF 3-6
strain: 129/Sv.
strain: BALB/c.
tissue: Embryonic stem cell.
8.

"Complementation of methylation deficiency in embryonic stem cells by a DNA methyltransferase minigene."
Tucker K.L. , Talbot D. , Lee M.A. , Leonhardt H. , Jaenisch R.
Proc. Natl. Acad. Sci. U.S.A.93:12920-12925(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-119 (ISOFORM 1)
strain: 129/Sv.
tissue: Embryonic stem cell.
tissue: Kidney.
9.

"The transcriptional landscape of the mammalian genome."
Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-272 (ISOFORM 1)
strain: C57BL/6J.
tissue: Embryo.
10.

"Activation of mammalian DNA methyltransferase by cleavage of a Zn binding regulatory domain."
Bestor T.H.
EMBO J.11:2611-2617(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 1108-1154;ENZYME REGULATION
11.

"Peptide mapping of the murine DNA methyltransferase reveals a major phosphorylation site and the start of translation."
Glickman J.F. , Pavlovich J.G. , Reich N.O.
J. Biol. Chem.272:17851-17857(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-515;IDENTIFICATION BY MASS SPECTROMETRY
tissue: Erythroleukemia.
12.

"DNA methyltransferase Dnmt1 associates with histone deacetylase activity."
Fuks F. , Burgers W.A. , Brehm A. , Hughes-Davies L. , Kouzarides T.
Nat. Genet.24:88-91(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HDAC1
13.

"DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci."
Rountree M.R. , Bachman K.E. , Baylin S.B.
Nat. Genet.25:269-277(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HDAC2 AND DMAP1
14.

"Genomic imprinting disrupted by a maternal effect mutation in the Dnmt1 gene."
Howell C.Y. , Bestor T.H. , Ding F. , Latham K.E. , Mertineit C. , Trasler J.M. , Chaillet J.R.
Cell104:829-838(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION
15.

"The activity of the murine DNA methyltransferase Dnmt1 is controlled by interaction of the catalytic domain with the N-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA."
Fatemi M. , Hermann A. , Pradhan S. , Jeltsch A.
J. Mol. Biol.309:1189-1199(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ALLOSTERIC REGULATION
16.

"Replication-independent chromatin loading of Dnmt1 during G2 and M phases."
Easwaran H.P. , Schermelleh L. , Leonhardt H. , Cardoso M.C.
EMBO Rep.5:1181-1186(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
17.

"The PHD finger/bromodomain of NoRC interacts with acetylated histone H4K16 and is sufficient for rDNA silencing."
Zhou Y. , Grummt I.
Curr. Biol.15:1434-1438(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH BAZ2A
18.

"The Polycomb group protein EZH2 directly controls DNA methylation."
Vire E. , Brenner C. , Deplus R. , Blanchon L. , Fraga M. , Didelot C. , Morey L. , Van Eynde A. , Bernard D. , Vanderwinden J.-M. , Bollen M. , Esteller M. , Di Croce L. , de Launoit Y. , Fuks F.
Nature439:871-874(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH THE PRC2 COMPLEX
19.

Vire E. , Brenner C. , Deplus R. , Blanchon L. , Fraga M. , Didelot C. , Morey L. , Van Eynde A. , Bernard D. , Vanderwinden J.-M. , Bollen M. , Esteller M. , Di Croce L. , de Launoit Y. , Fuks F.
Nature446:824-824(2006)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
20.

"Phosphorylation of serine-515 activates the mammalian maintenance methyltransferase Dnmt1."
Goyal R. , Rathert P. , Laser H. , Gowher H. , Jeltsch A.
Epigenetics2:155-160(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-515;MUTAGENESIS OF SER-515
21.

"Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation."
Schermelleh L. , Haemmer A. , Spada F. , Roesing N. , Meilinger D. , Rothbauer U. , Cardoso M.C. , Leonhardt H.
Nucleic Acids Res.35:4301-4312(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;MUTAGENESIS OF GLN-162; PHE-169 AND CYS-1229
22.

"Large-scale phosphorylation analysis of mouse liver."
Villen J. , Beausoleil S.A. , Gerber S.A. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152 AND SER-717;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Liver.
23.

"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M. , English L. , Lemieux S. , Courcelles M. , Desjardins M. , Thibault P.
Immunity30:143-154(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-717;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
24.

"Usp7 and Uhrf1 control ubiquitination and stability of the maintenance DNA methyltransferase Dnmt1."
Qin W. , Leonhardt H. , Spada F.
J. Cell. Biochem.112:439-444(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION;DEUBIQUITINATION BY USP7;INTERACTION WITH USP7 AND UHRF1
25.

"The DNA-binding activity of mouse DNA methyltransferase 1 is regulated by phosphorylation with casein kinase 1delta/epsilon."
Sugiyama Y. , Hatano N. , Sueyoshi N. , Suetake I. , Tajima S. , Kinoshita E. , Kinoshita-Kikuta E. , Koike T. , Kameshita I.
Biochem. J.427:489-497(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-146 BY CSNK1D/CK1;INTERACTION WITH CSNK1D
26.

"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J. , Chen Y. , Tishkoff D.X. , Peng C. , Tan M. , Dai L. , Xie Z. , Zhang Y. , Zwaans B.M. , Skinner M.E. , Lombard D.B. , Zhao Y.
Mol. Cell50:919-930(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-1122 AND LYS-1124;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic fibroblast.
27.

"Structure of DNMT1-DNA complex reveals a role for autoinhibition in maintenance DNA methylation."
Song J. , Rechkoblit O. , Bestor T.H. , Patel D.J.
Science331:1036-1040(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 731-1602 IN COMPLEX WITH SAH AND DNA;AUTOINHIBITORY LINKER

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